Microorganisms are too small to be seen with the unaided eye, therefore, they must be observed with a microscope. Due to the fast innovations and advances in microscopy, we are now capable of examining, determining and observing them. This experiment, which utilized the hay infusion as the residing area of microorganisms, investigated the living organism in a drop of water, their manifestation of the different attributes in life. During the run of the experiment, paramecium and specie 1 were observed and were tested to determine the impact of different stimulus to microorganisms in three setups. At the end of the experiment, the variety of setups has been shown to affect the ...view middle of the document...
Microorganisms, from the Greek word “mikros” meaning small, and “organismos”, which means organism, also spelled micro-organism, micro organism, microorganism or microbe is a microscopic organism that comprises either a single-cell(unicellular) or cell clusters. These include bacteria, fungi, archaea or protists, but not viruses and prions, which are generally classified as non-living. Microorganisms live almost everywhere on Earth where there is liquid water, including hot springs, on the ocean floor, and deep inside rocks within earth’s crust.
Under the microscope, the microorganism that we mostly found and easily noticed in this research were the paramecia of the Phylum Protozoa and specie 1 due to its observable movement and features.
Protozoa means “first-animal” which generally describes its animal-like nutrition. All protozoa live in areas with large supplies of water. However, Volk mentioned that even in the soil, protozoans live. They are unicellular, eukaryotic chemo heterotrophic organisms. Prescott defined Protozoa as Protists exhibiting heterotrophic nutrition and various types of locomotion. They occupy a vast array of habitats and niches and have organelles similar to those found in other eukaryotic cells and also specialized organelles.
Under this Phylum, is the free-living, non-pathogenic Paramecium which belongs to class Ciliata. Paramecium is a small one celled(unicellular) living organism that can move, digest food, and reproduce. They belong to the kingdom of Protista , which is a group(family) of similar living microorganisms. Microorganism means they are a very small living cell. The paramecia are known for their avoidance behavior, if it encounters a negative stimulus, it is capable of rotating up to 360 degrees to find an escape route. In addition to paramecium, the specie 1, that has a slender body structure and moves in a spiral-like motion, is another type of specie that is similar to a worm and always in close proximity to the debris but eventually leaves them .
In this paper, these organisms will likely to be discussed and will be tackled more as these organisms undergo several phases of concentration and analysis during the experiment.
Scope and Limitation of the Study
Due to the limitation of laboratory materials and time on this experiment, the incubation of the hay infusion had the run time of only five days and the organisms were observed for no more than six hours. This is probably the reason why there were limited species observed under the microscope namely the paramecium and specie 1. In addition, we are not able to identify all microorganisms on the different slide because of the limited knowledge and data about microbes.
II. MATERIALS AND METHODS
This study used cotton, dropper, glass slide, cover slip, microscope, brine solution, sugar solution and hay infusion.
In making hay infusion, any natural water will work except tap water. Tap water that we usually use is...