Measurement Of Ip3 In Cultured Cells

1157 words - 5 pages

Histamine which is an agonist induced an accumulation of inositol phosphate (IP3) in cultured cells both in the presence of calcium and in the absence of calcium. This mechanism of cellular response to the histamine receptor s a G-protein mediated activated (GTP) (Hill,1990).
This mechanism occurs when a response to a photon of light called rhodopsin causes a change in the G-protein which is associated. Transduction then occurs which makes the α subunit to replace GDP with a triphosphate GTP, this then separates from the βγ subunits. (Tuteja,2009).
Interaction of α subunit or the βγ complex with some particular isoforms of G-protein could excite phospholipase C (PLC) to ...view middle of the document...

2ml of neutralising solution was added to each vial, 800µl of sample was carefully taken from each well and added to the appropriate vial insert.
The content of the scintillation vial was inserted onto the appropriated matching column and allowed to drain through. The empty scintillation vial inserts was put in a waste bucket.

WELL NUMBER MEAN DPM1(IP3 accumulation) % RESPONSE LOG CONC.(histamine)
1 AND 2 486.035 26 -3
3 AND 4 1792.055 100 -4
5 AND 6 1069.510 60 -5
7 AND 8 788.220 44 -6
9 AND 10 385.480 22 -7
11 AND 12 336.775 19 -8
13 AND 14 234.774 78 -3
15 AND 16 134.421 100 -4
17 AND 18 60.896 81 -5
19 AND 20 43.367 73 -6
21 AND 22 139.051 50 -7
23 AND 24 4.533 37 -8

TABLE 1 shows the mean accumulation of IP3, percentage response ad log concentration of histamine of cells in 24 wells with 1-12 have calcium and 13-24 calcium free.
From the Table above, there is a positively significant difference in the mean IP3 accumulation between the experiment with calcium and the calcium free experiments.
The biggest difference can be seen at the highest log concentration of 10-3 Histamine, where mean DPM1 change for well with calcium is 1792.05 against that without calcium of 134.42. At concentration of 10-4, there is a difference of 259.54 in IP3 accumulation. As we look through the table there is significant difference in IP3 accumulation where calcium is absent and present

Graph A is a representation of the percentage response and histamine log concentration, with the sigmoidal curves indicating the experimental values with calcium and without calcium. There is a clear significant difference where the calcium free curve is lower down the graph.
Extrapolating the EC50. EC50 is a concentration of a drug that gives half maximum response.
EC50 of experiment with calcium is 10-6M.
EC50 of experiment without calcium is 10-4.5M.

Graph B illustrates the mean IP3 accumulation against histamine log concentration, where there is also a clear difference in the curves. This graph shows higher IP3 accumulation in presence of calcium as opposed to without calcium.
In this experiment, the histamine is the agonist and the G- Protein coupled receptors act to stimulate the production of IP3 in the presence and absence of calcium. According to the results obtained, there is a significant difference in the EC50 values of histamine in the presence and...

Other assignments on Measurement Of Ip3 In Cultured Cells

Perfomance Measures Essay

616 words - 3 pages Performance measurement is the process of using a tool based on research (a "performance measure") to evaluate a managed organization, plan or program. A responsible, providing entity can be identified, is held accountable for its observed behavior, and has a reasonable degree of control over the aspect of care being evaluated in what the term "performance" implies. The term "measurement" implies that the approach being used is rigorous and

Solar Cells And Plant Cells Compared

451 words - 2 pages Solar and Plant Cells Compared Tiffany L. Triggs Strayer University The Importance of Energy For plant Earth, the Sun is the main source for all energy. It is the main essential thing that provides us with light, warmth and energy. Some organisms, such as plants and algae, create their own food using the sun as their main source of energy. In turn the fuel the rest of the world by creating energy for animals and humans after being

Stem Cells

1194 words - 5 pages red blood cells can get damaged. These affected cells can easily be replaced with healthy stem cells that adapt. (Pros and cons of stem cell research, 1)The biggest debate about this topic isn’t about whether stem cells work, but who’s going to pay for the research. It’s not a matter of pro-life or pro-choice, it’s pro-science. If my family had the finances for stem cell therapy, we would have made sure that my grandmother was in as little as

Stem Cell Research

1172 words - 5 pages Katherine Soroj, a dermatologist, is suffering from Alzheimer’s. She has two sons and a daughter along with her loving husband. Her family has to see her suffer from the disease on the daily and they pray to God that one day she will be cured. With future research of stem cells, she can be cured. Stem cells are a large focus of research in today’s biomedical society. Stem cell research should be supported and funded by the United States. It is


409 words - 2 pages or are attached to membrane networks that snake their way through the cell--in either case, outside the nucleus. Gene mutations occur in two ways: they can be inherited from a parent or acquired during a person’s lifetime. Mutations that are passed from parent to child are called hereditary mutations or germ line mutations (because they are present in the egg and sperm cells, which are also called germ cells). This type of mutation is present

Stem Cell Treatment

726 words - 3 pages HUMN432 Team C Members Team Leader: Capriana Johnson Team Assistant Leader: Merrie Hill Team Editor: Lupe Jimenez Team member 1: Team member 2: Charles Jakeway Team member 3: Marvin Jones Outline Details Topic: Stem Cell Treatment in Cancer Introduction: (ends with the thesis) A brief description of the technology and an explanation of the associated science - Cornelious What are stem cells? Stem cells are immature cells

Prof Com

446 words - 2 pages or dead cells. B. Subpoint: Cancer cells continue to grow and divide. 1. Cancer cells accumulate in one place. 2. The accumulated cells form tumors. 3. Tumors destroy body cells. C. Subpoint: 2 types of tumors 1. Malignant 2. Benign

Ustralian And New Zealand Schemes During Global Financial Crisis

303 words - 2 pages place, a distinctive characteristic common in other developed nations. The Australian Government introduced a blanket guarantee on deposits namely the Australian Government Guarantee Scheme for Large Deposits and Wholesale Funding until October 2011. Consequently, it was cultured to a scheme in which the first AUD$ 1 million was to be guaranteed free of charge, with larger and foreign branch deposits able to be insured for a fee (Committee

Immune System

576 words - 3 pages Immune System-Part II The immune system is composed of a very complex network of organs, cells, and molecules. All of these contributors play a significant role in the maintaining the body’s homeostasis. There are two paths the immune system can take. These are known as innate and adaptive immunity. They work in conjunction with one another to provide the proper response to environmental factors. Innate immunity is characterized by a rapid

Cancer Immunology

2717 words - 11 pages Cancer: Cancer can be defining as disease condition in which cell divide and proliferate uncontrollably bypassing the normal rules of cell division. It is characterized by a progression of changes on cellular and genetic level that ultimately reprogram a cell to undergo uncontrolled cell division, thus forming a malignant mass. Carcinogenesis: Cancers consist of single clones or several clones of cells that are capable of

Stem Cell Research

2085 words - 9 pages Much like the decision made by former U.S. President Harry S. Truman in regards to the use of atomic energy against Japan in hopes of concluding the death toll of World War II in 1945, the controversy over stem cell development derives from the same question, just because we possess the ability, does that justify its use? Scientists believe the process of extracting and cultivating stem cells for medical use may unlock the cure for countless

Similar Documents

Isolation Of Rare Circulating Tumour Cells In Cancer Patients

7750 words - 31 pages provides a new and effective tool for accurate identification and measurement of CTCs in patients with cancer. It has broad implications in advancing both cancer biology research and clinical cancer management, including the detection, diagnosis and monitoring of cancer10. CTCs are rare, comprising as few as one cell per 109 haematologic cells in the blood of patients with metastatic cancer, hence their isolation presents a tremendous technical

Stem Cell Funding Essay

933 words - 4 pages Stem Cell Funding Stem cell research has been a controversial hot topic in court and in the lab. The controversy involved with stem cell research starts with the means of obtaining the cells to perform the research. These stem cells are often obtained from deceased embryos of human beings. Ethics and moral issues arise because of the abortions occurring solely to acquire the coveted stem cells. Stem cells are basically cells that are capable

Apple Inc Essay

4956 words - 20 pages UNIT 6 ATTITUDE MEASUREMENT AND SCALES Objectives After reading this unit, you should be able to : • • • • describe the type of managerial research problems which utilise the tools of attitude measurement; discuss the type of issues which come up when one attempts the measurement of attitudes; explain the different attitude measurement scales, alongwith their strengths and limitations; decide for which type of research problems one should go in

Stem Cell Research Paper

1283 words - 6 pages SBI4U ISP Part II: Genetic Technology Research Paper Embryonic Stem Cell Use Abstract: Researchers discovered that embryonic stem cells could be originated from early human embryos. This discovery has raised a series of ethical and public-policy questions that are now being confronted by multiple international organizations, nations, cultures, and religious traditions. This essay surveys policies for human embryonic stem cell research in